Textbook in PDF format This textbook, designed for all scientists interested in protein research, provides a thorough overview of laboratory methods for the biophysical chemistry of proteins. This new edition, completely restructured and expanded for ease of learning, includes sections on analytical techniques, working with proteins, protein size and shape, protein structure, enzyme kinetics, industry enzymology, and a new section on special statistics. Analytical Techniques Microscopy Principles of Optical Spectroscopy Electrophoresis Immunological Methods Isotope Techniques Working with Proteins Purification of Soluble Proteins Membrane Proteins Determination of Protein Concentration Cell Culture Protein Modification Protein Size and Shape Centrifugation Molecules in Solution Non-resonant Interactions with Electromagnetic Waves Protein Structure Protein Sequencing Protein Secondary Structure Tertiary Structures Folding and Unfolding of Proteins Enzyme Kinetics Steady-State Kinetics Reaction Velocities Protein–Ligand Interactions Industrial Enzymology Immobilised Enzymes Special Statistics Quality Control Testing Whether or Not a Model Fits the Data Presenting Data