Textbook in PDF format Biophysical Chemistry explores the concepts of physical chemistry and molecular structure that underlie biochemical processes. Ideally suited for undergraduate students and scientists with backgrounds in physics, chemistry, or biology, it is also equally accessible to students and scientists in related fields as the book concisely describes the fundamental aspects of biophysical chemistry and puts them into a biochemical context. This second edition has been fully updated throughout with novel techniques, with a new chapter on advances in cryo-electron microscopy and exciting new content throughout on big data techniques, structural bioinformatics, systems biology and interaction networks, and artificial intelligence and machine learning. The book is organized in four parts, covering thermodynamics, kinetics, molecular structure and stability, and biophysical methods. Cross-references within and between these parts emphasize common themes and highlight recurrent principles. End of chapter problems illustrate the main points explored and their relevance for biochemistry, enabling students to apply their knowledge and to transfer it to laboratory projects. Key Features: Connects principles of physical chemistry to biochemistry. Emphasizes the role of organic reactions as tools for modification and manipulation of biomolecules. Includes a comprehensive section on the theory of modern biophysical methods and their applications. Systems, state functions, and the laws of thermodynamics Energetics and chemical equilibria Statistical thermodynamics Thermodynamics of transport processes Electrochemistry Reaction velocities and rate laws Integrated rate laws for uni- and bimolecular reactions Reaction types. Deriving integrated rate laws by solving sets of differential equations with matrix algebra Rate-limiting steps Binding reactions: One-step and two-step binding Single-molecule kinetics Steady-state (enzyme) kinetics Complex reaction schemes and their analysis Temperature dependence of rate constants Principles of catalysis Molecular structure and interactions Proteins Nucleic acids Macromolecular modeling Optical spectroscopy Magnetic resonance Solution scattering Crystallography Fluorescence imaging and microscopy Electron microscopy Scanning probe microscopy and force measurements Transient kinetic methods Molecular mass, size, and shape Calorimetry Mathematical concepts used in this book